A new technique for photographing perineal patterns of root-knot nematodes. populations obtained, M. incognita and M. javanica… 2018-02-19T11:36:17Z The double lateral lines are characteristic. The morphology of the adult female is usually the most important character in the identification of species of Meloidogyne (root-knot nematodes) (2,9,11,17,23,24,31). 2018-02-19T11:36:16Z converted Their detection requires larger amounts of homogenates from several females. Useful morphological characters for the separation of M. mayaguensis from M. incognita from Florida are the male stylet length values (smaller for M. mayaguensis than M. incognita) and J2 tail length values (greater for M. mayaguensis than M. incognita). U. Meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance. Enzyme analyses of Florida M. mayaguensis isolates show two major bands (VS1-S1 phenotype) of esterase activity, and one strong malate dehydrogenase band (Rm 1.4) plus two additional weak bands that migrated close together. Clipboard, Search History, and several other advanced features are temporarily unavailable. Allee. Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. Perineal patterns of all nine populations were observed with a LM and then examined with a SEM. Nucleotide sequence comparison of these mitochondrial products together with sequence from 18S rDNA and ITS1 from the nuclear genome were nearly identical with the corresponding regions from a M. mayaguensis isolate from Mayaguez, Puerto Rico, the type locality of the species. stressful conditions. cause yield loss in all countries, of which, M. javanica, is the most widespread species in Iran. In addition, female stylets of each population were excised, viewed with a SEM, and compared with observations made with a LM. There are over 13,543,000 records available in CAB Direct | Last updated on October 20, 2020. Drake. resistance is not available). Species is often dominant at higher altitudes in warm climates.. and Mullin, {P. G.} and Inserra, {R. N.} and Dickson, {D. W.}". typical of genus. Enzyme analyses of Florida M. mayaguensis isolates show two major bands (VS1-S1 phenotype) of esterase activity, and one strong malate dehydrogenase band (Rm 1.4) plus two additional weak bands that migrated close together. Meloidogyne javanica is a species of plant-pathogenic nematodes. Nucleotide sequence comparison of these mitochondrial products together with sequence from 18S rDNA and ITS1 from the nuclear genome were nearly identical with the corresponding regions from a M. mayaguensis isolate from Mayaguez, Puerto Rico, the type locality of the species. Meloidogyne mayaguensis values for these characters overlap with those of M. arenaria and M. javanica from Florida. It is one of the tropical root-knot nematodes and a major agricultural pest in many countries. The SEM emphasized surface details, whereas the LM revealed subcuticular structure as well. You can now claim your publications on CAB Direct with your ORCID iD! | %PDF-1.5 %���� Amplification of two separate regions of mitochondrial DNA resulted in products of a unique size. Morphological Comparison of Second-Stage Juveniles of Six Populations of Meloidogyne hapla by SEM. Abstract: The external morphology of female heads of three populations of each of two cytological races of Meloidogyne hapla (race A--meiotic, race B--mitotic) and single populations of M. arenaria, M. incognita, and M. javanica was compared by light (LM) and scanning electron proof:pdf along its length. Like most websites we use cookies. PCR primers embedded in the COII and 16S genes produced a product size of 705 bp, and amplification of the 63-bp repeat region resulted in a single product of 322 bp. Amplification of two separate regions of mitochondrial DNA resulted in products of a unique size. The perineal patterns of M. mayaguensis isolates from Florida show morphological variability and often are similar to M. incognita. Meloidogyne enterolobii and M. javanica were identified from U. parvifolia cv. Allee and Drake in Lake County, Florida. PCR primers embedded in the COII and 16S genes produced a product size of 705 bp, and amplification of the 63-bp repeat region resulted in a single product of 322 bp. Molecular techniques for the identification of M. mayaguensis will be particularly useful in cases of M. mayaguensis populations mixed with M. arenaria, M. incognita, and M. javanica, which are the most economically important root-knot nematode species in Florida, and especially when low (<25) numbers of specimens of these species are recovered from the soil. title = "Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida". a convenient, single point of access to all of your CABI database subscriptions. Molecular techniques for the identification of M. mayaguensis will be particularly useful in cases of M. mayaguensis populations mixed with M. arenaria, M. incognita, and M. javanica, which are the most economically important root-knot nematode species in Florida, and especially when low (<25) numbers of specimens of these species are recovered from the soil. Morphological Comparison of Three Host Races of Meloidogyne javanica. NIH incorporating the leading bibliographic databases CAB Abstracts and Global Health. Nematode species were identified using both molecular analysis and morphology of perineal patterns. There is less plant resistance to attack by this species not confer resistance to M. incognita; that had to be provided from other Rotation alternatives: For an extensive host range list for this They may develop in larger numbers under PCR primers embedded in the COII and 16S genes produced a product size of 705 bp, and amplification of the 63-bp repeat region resulted in a single product of 322 bp. Amplification of two separate regions of mitochondrial DNA resulted in products of a unique size. If you would like to, you can learn more about the cookies we use. from application/x-indesign to application/pdf Meloidogyne mayaguensis reproduced on cotton, pepper, tobacco, and watermelon but not on peanut. Get the latest research from NIH: https://www.nih.gov/coronavirus. Morphology and Anatomy: Sample perineal patterns of Meloidogyne javanica. The external morphology of female heads of three populations of each of two cytological races of Meloidogyne hapla (race A-meiotic, race B-mitotic) and single populations of M. arenaria, M. incognita, and M. javanica was compared by light (LM) and scanning electron microscopy (SEM). A morphological and morphometric comparison using light microscopy and scanning electron microscopy was made of 6 populations of Meloidogyne javanica belonging to 3 host races (infective on pepper (Capsicum frutescens), peanut (Arachis hypogaea), or noninfective on both). Meloidogyne mayaguensis values for these characters overlap with those of M. arenaria and M. javanica from Florida. Among the Meloidogyne spp. parameters, Length of rotation of Ermelo Weeping Lovegrass (, For plants reported to have some level of resistance to this species, click. False Crop Molecular techniques for the identification of M. mayaguensis will be particularly useful in cases of M. mayaguensis populations mixed with M. arenaria, M. incognita, and M. javanica, which are the most economically important root-knot nematode species in Florida, and especially when low (<25) numbers of specimens of these species are recovered from the soil. Dive into the research topics of 'Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida'. Continuing to use www.cabdirect.org Hosts Meloidogyne javanica is a nematode pathogen that affects over 770 species of plants (Cabi 2018). COVID-19 is an emerging, rapidly evolving situation. Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Perineal-pattern morphology of female specimens is one of the most common characteristics used for identification. Body shapes of immature and mature females. Cultivars of carrot derived from / Brito, J.; Powers, T. O.; Mullin, P. G.; Inserra, R. N.; Dickson, D. W. T1 - Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. session so others can sign in. life processes of M. xmp.did:12EBFCA4DC1FE5118C94CD2E8B2F434D Preliminary results indicate the M. mayaguensis isolates from Florida can reproduce on tomato containing the Mi gene. Among the Meloidogyne spp. Find out more about this exciting new development, Using our new visualization tools you can, Using our new highlighting and annotation tool you can, remove selected records that are not saved in My CABI, sign you out of your M. arenaria; M. incognita; M. javanica; Meloidogyne hapla; cytological races; root-knot nematodes; scanning electron microscopy. species was M. incognita Morphological Comparison of Head Shape and Stylet Morphology of Second-stage Juveniles of Meloidogyne Species. session so others can sign in. hours). endstream endobj 5 0 obj <> endobj 3 0 obj <> endobj 7 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 8 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 9 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 10 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 11 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 12 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/TrimBox[21.0 21.0 587.929 814.701]/Type/Page>> endobj 40 0 obj <>stream Enzyme analyses of Florida M. mayaguensis isolates show two major bands (VS1-S1 phenotype) of esterase activity, and one strong malate dehydrogenase band (Rm 1.4) plus two additional weak bands that migrated close together. Meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance.
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